ࡱ > %` bjbj"x"x 4: @ @ . . . . . . . B j j j j ~ B 5 7 7 7 7 7 7 $ l h [ . " [ . . S p . . 5 5 . . YH j : 5 0 ^ Y . ^ 8 ^ . ` [ [ B B B D B B B B B B . . . . . . Wolfson Centre for Inherited Neuromuscular Disease ===============CIND=============== Product Datasheet for Monoclonal Antibody Name: MANDYS126 - MOUSE ANTI-DYSTROPHIN Clone No. 3E5C9 Specificity: Dystrophin Epitope: Ex38: aa-F-SDI--L--P Species: human, mouse, dog, xenopus (not chick, torpedo) Isotype: IgG1 Western blot: Stains a 427kD in normal human muscle extract. Dilution: 1/100 Human muscle extract. Normal human muscle section Immunolocalization: Weak staining of muscle membranes Frozen, unfixed sections. May not work on formalin-fixed tissue. Dilution: 1/4 Immunogen: Recombinant human dystrophin amino-acids 1749-2248 as TrpE fusion protein Storage recommendation: Store one month's use at 4oC and remainder in aliquots at 20oC. Stable for at least 12 months under these conditions Concentration: estimated at >40micrograms per ml (not measured directly) Supplied as liquid culture supernatant in DMEM/10% horse serum (contains AZIDE: toxic!) (Identical culture medium can be supplied as negative control) References: 1/ Monoclonal antibodies against defined regions of the muscular dystrophy protein, dystrophin. Nguyen Thi Man, A.J.Cartwright, G.E.Morris, D.Love, J.F.Bloomfield and K.E.Davies. FEBS Letters, 262 (1990) 237-240. 2/ Use of epitope libraries to identify exon-specific monoclonal antibodies for characterization of altered dystrophins in muscular dystrophy. Nguyen Thi Man and G.E.Morris, American J.Human Genetics, 52 (1993) 1057-1066. 3 5 X Y o % ' * . 1 : K L S T _ l m / 0 1 D q r ɾ·ݰݰݰ݆tp hK_ j hlO hlO Uh h hH h CJ OJ QJ ^J aJ h CJ OJ QJ ^J aJ hh hh hh h h h\` h hO h\` h\` h\` h } h9 h+ h 5h h CJ h 5hu/ 5CJ aJ hu/ 5 hu/ CJ ' 3 X Y o m n 0 3 w gd $]a$gd gd ]gd ]gdu/ gdu/ r s w 5 6 Y d f 6 ɽ h^e hKp h\` hlL h H*h hh h 4 hvf hvf h huw h h j hK_ hvf U 9 r w X Y < gdlL ]gd 2 1h:p . A!"#$% `! ZxFCbS D G ( t/ xzgsW%HAz LJX-K5rX윻فh[s9s53?Sw3OE }p=99,!# ;߄#?"gLT4ŷ_$ۿ;/Oޅf!yo{Y I^/"yN>O~3?N>яO}S#>Fw}??Lw|'Ї>D7Mo}+g?K>O7d|_ '>ri/@!_җ>9pGΞ=K>ϓ{|_$o~[ {_z7ܺuկ&/yk^C '˲ȍ7͛7,DQ:}k'|Kl&aqi&/+^ ru*$H^W]K.+WG}<3駟&|+SO=E~CW'x<#/_&> yGŋɅȳ>Kx'|Nկ}k+_ N~67Ioo}[?G'?1/~A~?)~F~_5o~C~_?wL~ߓ+H?/园Z2R*2.ҥ(OHM x+ϖ3`.gT˴Xk^NU*#2Qȴh4բ1DzTNR<8jz zS\Xݏz3\nZL%c!ȵlSkכQ!&zc*xDM=X#ƄV= -syEz"Γzj. M'i$(z:ŞʱEM%SRudU7%]ME|TuUEBl꒡*"ˊIs^Ҡjy+i:~@E*)ncphnHkɒbX;xyXKe莧ؖ-keh-'+t MIv7W4Voll= CLz#ŲxܳR|ͶnzsOL3ܛ'鸊gs?7}% ec m]uMו=k.Ε=S|`>}f7w`cݘC&-t<eoJjz87 kglP7mS5w \Xg(+ᇶنw{'$\eӒ}[] |ahܐ ;x40rMɁTxϖ=7n3\<3,W}՜]'p C<ZV َu`^ULjyː